스킵네비게이션

분자생명공학 연구실

담당교수: 이대원 교수
분자생명공학연구실 (담당교수: 이대원)

실험실 위치: 건학기념관 (30호관 503호)

실험실 소개
21세기는 바이오의 시대이면서 한편으로 생명안전이라는 큰 아젠다가 중요한 시대입니다. 새로운 생명현상을 이해하고, 이를 통해 생명안전에 기여할 수 있는 분야를 연구합니다. 보이지 않는 분자수준의 연구를 통해, 도전적이면서 창의와 열정을 가진 생명공학자의 꿈을 키워가는 곳입니다.

주관심 분야
기능성 대사체, 분자진단 (항체 및 유전자), 분자마커, 유전체학, 곤충페로몬

최근 연구과제(연구책임)
■ 영남지역 벼재배 지대별 비래해충 실태조사 및 발생특성 분석 (농촌진흥청-3.3억/ 2016-2020)
■ 담배가루이 유인트랩 및 친환경 해충방제기술개발 (농촌진흥청-10.2억/ 2021-2023)
■ 기능성 소재 대사체연구지원센터 (교육부 Core facility (1단계)- 20.5억/ 2019-2022)

최근 연구논문 (SCI급)
2018-현재
■ W.H. Cha, J.K. Jung, Y.G. Kim, D.-W. Lee. 2018. Identification and pheromonotropic activity of pheromone biosynthesis activating neuropeptide in Maruca vitrata. J. Asia-Pacific Entomol. 21: 156-160.
■ W.H. Cha, D.-W. Lee. 2018. RNA interference of trehalose phosphate synthase inhibits metamorphosis and decreases cold tolerance in the diamondback moth, Plutella xylostella (L.). J. Asia-Pacific Entomol. 21: 1034-1039.
■ W.H. Cha, J.K. Jung, D.-W. Lee. 2018. Identification of G-protein coupled receptors from the pheromone gland of Maruca vitrata by tranx-x-x-scriptomic analysis. J. Asia-Pacific Entomol. 21: 1203-1210.
■ M.A. Al Baki, D.-W. Lee, J.K. Jung, Y. Kim. 2019. Insulin-like peptides of the legume pod borer, Maruca vitrata, and their mediation in hemolymph trehalose level, larval development, and adult reproduction. Arch. Insect Biochem. Physiol. 100: e21524
■ M.A. Al Baki, D.-W. Lee, J.K. Jung, Y. Kim. 2019. Insulin signaling mediates oogenesis and enhances vitellogenesis induced by juvenile hormone in a lepidopteran insect, Maruca vitrata. BMC Biology 19:14.
■ W.H. Cha, D.-W. Lee. 2019. C-terminal conserved motifs in neprilysin are not required for host immune suppression. J. Asia-Pacific Entomol. 22: 1161-1166.
■ H. Kwon, Y. Yang, S. Kumar, D.-W. Lee, P. Bajracharya, T.L. Calkins, Y. Kim, P.V. Pietrantonio. 2020. Characterization of the first insect prostaglandin (PGE2) receptor: MansePGE2R is expressed in oenocytoids and lipotheicoic acid (LTA) increases tranx-x-x-script expression. Insect Biochem. Mol. Biol. 117: 103290.
■ A.B. Md Abdullaha, D.-W. Lee, J. Jung, Y. Kim. 2020. Deletion mutant of sPLA2 using CRISPR/Cas9 exhibits immunosuppression, developmental retardation, and failure of oocyte development in legume pod borer, Maruca vitrata. Develop. Comp. Immunol. 103:103500
■ S. Ahmed, M.A.A. Baki, J. Lee, D.Y. Seo, D.-W. Lee, Y. Kim. 2021 The first report of prostacyclin and its physiological roles in insects. Gen. Comp. Endocrinol. 301: 113659
■ Y.‑C. Kim, J. Lee, D.‑W. Lee, B.‑H. Jeong. 2021. Large-scale lipidomic profling identifes novel potential biomarkers for prion diseases and highlights lipid raft-related pathways. Vet. Res. 52:10
■ S. Ahmed, M.C. Roy, Md. A. Al. Baki, J.K. Jung, D. Lee, Y. Kim. 2021. CRISPR/Cas9 mutagenesis against sex pheromone biosynthesis leads to loss of female attractiveness in Spodoptera exigua, an insect pest. PLoSOne 16: e0259322
■ D.-W. Lee. 2022. Identification of neuropeptide receptors from the brain of the Bean pod borer, Maruca vitrata. J. Asia-Pacific Entomol. 25: 101845    
■ W.H. Cha, D.-W. Lee. 2022. Suppression of pheromone biosynthesis and mating behavior by RNA interference of pheromone gland-specific fatty acyl reductase in Maruca vitrata. Insect Science 29: 1135-1144
■ J. Lee, D.-W. Lee, W.H. Cha. 2022. Multiple precursor proteins of thanatin isoforms, an antimicrobial peptide associated with the gut symbiont of Riptortus pedestris. Front. Microbiol. 12: 796548    
■ W.H. Cha, D.-W. Lee. 2022. Suppression of glycerol biosynthesis-related genes decreases the effect of rapid cold hardening in Helicoverpa assulta. Entomol. Res. 52: 24-32  
■ J. Lee, D.-W. Lee. 2022. Insecticidal serralysin of Serratia marcescens is detoxified in M3 midgut region of Riptortus pedestris. Front. Microbiol. 13:913113.       
■ J. Lee, B. Lee, J.-J. Park, I. H. Jeong, D.-W. Lee. 2022. Loop-mediated Isothermal Amplification of PBAN gene for Molecular Diagnosis of Bemisia tabaci Biotype Q (Hemiptera: Aleyrodidae). J. Asia-Pacific Entomol. 25: 101942    
■ J. Lee, D.-W. Lee. 2022. Burkholderia gut symbiont induces insect host fecundity by modulating Kr-h1 gene expression. Arch. Insect Biochem. Physiol. 112: e21987
■ W.H. Cha, B. Kim, D.-W. Lee. 2023. Functional analysis of pheromone biosynthesis activating neuropeptide receptor isoforms in Maruca vitrata. Cells 12: 1410. 
■ M. Mannaa, A. Mansour, I. Park, D.-W. Lee, Y.-S. Seo. 2023. Insect-based agri-food waste valorization: Agricultural applications and roles of insect gut microbiota. Environ. Sci. Ecotechnol. 17: 100287
■ W.H. Cha, Y.E. Na, D.-W. Lee. 2023 Identification of immune-related genes from tranx-scriptome of the venom gland in Cotesia plutellae. Entomol. Res.   (In Press)

실험실 구성원
■ 담당교수 : 이대원
■ 연구전담교수 : 이준범, 차욱현
■ 대학원생 연구원 : 이병희, 박지현
■ 학부 연구생 : 박지현, 안승현, 오유정, 장귀원